Coding

Part:BBa_K2029007:Design

Designed by: Bui Hoang Duy   Group: iGEM16_UBonn_HBRS   (2016-10-14)


EstC2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 778


Design Notes

C-terminal 6His-tag added for convenient purification


Source

https://www.ncbi.nlm.nih.gov/nucleotide/283462288?report=genbank&log$=nuclalign&blast_rank=1&RID=XKTEYKV5014&from=7&to=1710

References

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139797/